Remove the vials from the freezer
and evaporate off petroleum ether under N2.
Suspend methylated
fatty acids
in isooctane [Sample 1(L&T: 200microliter) Sample 2(J&K: 500microliter),
which is the carrier solvent for the the gas chromatograph.
Inject 1 mL
of each sample in the gas chromatograph (HP
5890).
Assess lipid
composition by comparing to retention times of known methylated fatty
acids.