Grant                        Lincoln Public Schools Foundation Grant:  LHS/UNL Collaboration Research Experience

                                 for Advanced Chemistry Students.

                                    PDF Format

Participants

2001-2002 LHS Advanced Chemistry Class

L-R.  Nghia Le, Mr. Sean Putnam, Dimi Hua, Kristin Williams, 

Lien Phun, Angela Maske, Huong Le, Tri Huynh. 

Not pictured. Jenny Truong

Itinerary

Friday, April 26^th.

            8:30 Arrive at UNL, Plant Industry.  Orientation, Project Outline & Prep Insect for Dissection.

            8:45 Introduction by Dr. Stanley.  Insects as models for physiological & biochemical research.

            9:00 Dissection, Protein Extraction/Analysis & Lipid Extraction

                        -Team 1: Prostaglandin Biosynthesis

                        -Team 2: Fatty Acid Analysis

            10:45 Prostaglandin Biosynthesis & Isolate Lipid Sample (TLC)

            12:00 Lunch:  Plant Industry Meeting Room.

            12:20 Seminar: Physiology of Prostaglandins.  Dr. Stanley

            12:45 Extraction of Prostaglandin samples & Methylation of Lipids

Saturday, April 27^th.

            9:45 Recap experiment & overview of chromatography

10:00 Samples preparation for chromatography. Diazomethane & BSTFA reactions with PGs

            10:30 Begin running standards on GC & GC/MS

            12:45 Begin running samples on GC & GC/MS

            12:00 Lunch. On our own.

            12:30 Seminar: How insect immunity correlates mammalian immunity

            1:00 Finish sample analysis and data processing